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Anti-Cyclin-dependent kinase 4 Cdk4 Antibody Picoband®

Anti-Cyclin-dependent kinase 4 Cdk4 Antibody Picoband®

Item no.	BOS-PA1428-1
Manufacturer	Boster
Amount	100 ug/vial
Category	Oncology Neuroscience Neural Lineage Markers Antibodies By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Status Western Blot Proliferation Primary Antibodies By Organ Lung Cancer Skin Cancer Bladder Cancer
Type	Antibody Polyclonal
Format	Lyophilized
Applications	WB, FC, IF, ICC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Sensitivity	>5000 cells
Citations	1. Demetrick, D. J.; Zhang, H.; Beach, D. H. : Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes. Cytogenet. Cell Genet. 66: 72-74, 1994. 2. Modiano, J. F.; Mayor, J.; Ball, C.; Fuentes, M. K.; Linthicum, D. S. : CDK4 expression and activity are required for cytokine responsiveness in T cells. J. Immun. 165: 6693-6702, 2000.e for human proliferating nuclear antigen/cyclin by in situ hybridization. Hum. Genet. 86: 84-86, 1990.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Cyclin-dependent kinase 4;2.7.11.22;CRK3;Cell division protein kinase 4;PSK-J3;Cdk4;Crk3;
Similar products	Cdk 4 antibody, cdk4 antibody, CDK4 protein antibody, CDK4_HUMAN antibody, Cell division kinase 4 antibody, Cell division protein kinase 4 antibody, CMM 3 antibody, CMM3 antibody, Crk3 antibody, Cyclin dependent kinase 4 antibody, Cyclin-dependent kinase 4 antibody, Melanoma cutaneous malignant 3 antibody, MGC14458 antibody, p34 cdk4 antibody, PSK J3 antibody, PSK-J3 antibody
Available
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	34 kDa
Calculated Molecular weight	33751 MW
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5?g/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 ?g/ml, Human Flow Cytometry (Fixed), 1-3 ?g/1x10⁶ cells, Human
Manufacturer - Gene Name	CDK4
Gene Full Name	Cyclin-dependent kinase 4
Background	Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cdk4, different from the related rat sequence by one amino acid, and from the related human sequence by four amino acids.
Contents	Each vial contains 5 mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Cancer, Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases
Protein Name	Cyclin-dependent kinase 4
Protein Function	Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G (1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).
Subcellular Localization	Cytoplasm. Nucleus. Membrane. Cytoplasmic when non- complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G (1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G (1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus (By similarity).
Description	Boster Bio Anti-Cyclin-dependent kinase 4 Cdk4 Antibody catalog # PA1428-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines.
Sequence Similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-PA1428-1-datasheet.pdf

BOS-PA1428-1-safety-datasheet.pdf