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Anti-Transcription factor E2F1 E2F1 Antibody Picoband®

Anti-Transcription factor E2F1 E2F1 Antibody Picoband®

Item no.	BOS-PA1560
Manufacturer	Boster
Amount	100 ug/vial
Category	Oncology Neuroscience Neural Signaling Antibodies By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Status Western Blot Senescence Primary Antibodies Astrocytoma By Organ Bladder Cancer Head & Neck Cancer Bone Cancer
Type	Antibody Polyclonal
Format	Lyophilized
Applications	WB
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Sensitivity	0.21 ng/ml
Citations	1. Neuman E, Sellers WR, McNeil JA, Lawrence JB, Kaelin WG Jr (Dec 1996). "Structure and partial genomic sequence of the human E2F1 gene". Gene 173 (2): 163–9. 2. Morris, E. J., Ji, J.-Y., Yang, F., DiStefano, L., Herr, A., Moon, N.-S., Kwon, E.-J., Haigis, K. M., Naar, A. M., Dyson, N. J. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. Nature 455: 552-556, 2008. 3. Neuman, E., Sellers, W. R., McNeil, J. A., Lawrence, J. B., Kaelin, W. G., Jr. Structure and partial genomic sequence of the human E2F1 gene. Gene 173: 163-169, 1996.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Transcription factor E2F1;E2F-1;PBR3;Retinoblastoma-associated protein 1;RBAP-1;Retinoblastoma-binding protein 3;RBBP-3;pRB-binding protein E2F-1;E2F1;RBBP3;
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Available
Specificity	No cross reactivity with other proteins.
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	60 kDa
Calculated Molecular weight	46920 MW
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5?g/ml, Human , Mouse, Rat
Manufacturer - Gene Name	E2F1
Gene Full Name	Transcription factor E2F1
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family of transcription factors appears to play a critical role in the transcription of certain genes required for cell cycle progression. E2F1, the first cloned member of this family, is regulated during the cell cycle at the mRNA level by changes in transcription of the E2F1 gene and at the protein level by complex formation with proteins such as the retinoblastoma gene product (pRB), cyclin A and DP1. E2F1 can override a pRB-induced G1/S block and can behave as an oncogene in certain cells. E2F1 was cloned and was found to contain seven exons. Fluorescence in situ hybridization localized E2F1 to chromosome 20q11. The E2F1 transcription factor can promote proliferation or apoptosis when activated, and is a key downstream target of the retinoblastoma tumour suppressor protein (pRB).
Immunogen	A synthetic peptide corresponding to a sequence in the middle region of human E2F1, identical to the related mouse and rat sequences.
Contents	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Domain Families, Epigenetics and Nuclear Signaling, Forkhead Box, Oncoproteins/Suppressors, Transcription, Tumor Suppressors
Protein Name	Transcription factor E2F1(E2F-1)
Protein Function	Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812).
Subcellular Localization	Nucleus.
Description	Boster Bio Anti-Transcription factor E2F1 E2F1 Antibody catalog # PA1560. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	All of the tissues tested and carcinomas.
Sequence Similarities	Belongs to the E2F/DP family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-PA1560-datasheet.pdf

BOS-PA1560-safety-datasheet.pdf