Comparison

Anti-Osteopontin/SPP1 Antibody Picoband® European Partner

Item no. BOS-RP1080
Manufacturer Boster
Amount 100 ug/vial
Quantity options 100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category
Type Antibody Polyclonal
Format Lyophilized
Applications WB, ELISA
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
Sensitivity <1 pg/ml
Sequence Belongs to the osteopontin family.
Citations 1. Entrez Gene: SPP1 secreted phosphoprotein 1.
2. Reinholt FP, Hultenby K, Oldberg A, Heinegård D (June 1990). Osteopontin--a possible anchor of osteoclasts to bone. Proc. Natl. Acad. Sci. U.S.A. 87 (12): 4473–5.
3. Wang KX, Denhardt DT (2008). Osteopontin: role in immune regulation and stress responses. Cytokine Growth Factor Rev. 19 (5-6): 333–45.
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Osteopontin,Bone sialoprotein 1,Nephropontin,Secreted phosphoprotein 1,SPP-1,Urinary stone protein,Uropontin,SPP1,BNSP,OPN,PSEC0156
Available
Manufacturer - Category
Primary Antibodies
Storage Conditions
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight
66 kDa, 25-55 kDa
Calculated Molecular weight
35423 MW
Clonality
Polyclonal
Application Details
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, -
ELISA, 0.1-0.5μg/ml
Gene Name
SPP1
Gene Full Name
Osteopontin
Manufacturer - Specificity
No cross reactivity with other proteins.
Background
Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP1), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. And the encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. Also, this protein is a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Osteopontin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by seven amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category
Angiogenesis, Cancer, Cardiovascular, Cytoskeleton/ECM, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells, Structures, Tumor Biomarkers
Protein Name
Osteopontin
Protein Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Subcellular Localization
Secreted.
Description
Boster Bio Anti-Osteopontin/SPP1 Antibody catalog # RP1080. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity
Bone. Found in plasma.
Sequence Similarities
Belongs to the osteopontin family.
Manufacturer - Cross Reactivity
No cross-reactivity with other proteins

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug/vial
Available: In stock
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