Manufacturer |
Boster
|
Category |
|
Type |
Antibody Monoclonal |
Specific against |
Human, Mouse, Rat, Pig |
Clone |
NR4 |
Isotype |
IgG1 |
Format |
Lyophilized |
Applications |
WB, IHC, IHC-F |
Amount |
100 ug/vial |
Host |
Mouse |
Item no. |
BOS-MA1070 |
Targets |
NEFL |
eClass 6.1 |
32160702 |
eClass 9.0 |
42030590 |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Mouse Monoclonal Antibodies |
Manufacturers Research Category |
Cell Type Marker, Cytoskeleton, Cytoskeleton/ECM, Developmental Biology, Intermediate Filaments, Lineage Markers, Lineage Specification, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction, Stem Cells |
Short Description |
Boster Bio Anti-NF68 Nefl Antibody (Monoclonal, NR4) catalog # MA1070. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat. |
Description |
Boster Bio Anti-NF68 Nefl Antibody (Monoclonal, NR4) catalog # MA1070. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat. |
Background |
Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM) and 200 kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament Protein, Light Chain (NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons. |
Gene Name |
NEFL |
Application Details |
Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, pig, rat, By Heat Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, mouse, pig, rat, - Western blot, 1-2μg/ml, Human, mouse, pig, rat |
Application Notes |
Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Monoclonal |
Concentration |
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
Contents |
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
Immunogen |
Pig spinal cord. |
Purification |
Ascites |
Cross-reactivity |
No cross-reactivity with other proteins |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
Gene Full Name |
Neurofilament light polypeptide |
Molecular Weight |
61335 MW |
Protein Function |
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. |
Protein Name |
Neurofilament light polypeptide |
Sequence Similarities |
Belongs to the intermediate filament family. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.