Comparison

Anti-Apolipoprotein E/APOE Antibody Picoband® European Partner

Item no. BOS-PB9327
Manufacturer Boster
Amount 100 ug/vial
Category
Type Antibody Polyclonal
Format Lyophilized
Applications WB, FC, IF, IHC, ELISA
Specific against Human (Homo sapiens)
Host Rabbit
Isotype IgG
Sensitivity <5pg/ml
Citations 1. Calabuig-Navarro MV, et al. Apolipoprotein E genotype has a modest impact on the postprandial plasma response to meals of varying fat composition in healthy men in a randomized controlled trial. J Nutr, 2014 Nov.
2. APOE ? variants increase risk of warfarin-related intracerebral hemorrhage.Falcone GJ, et al. Neurology, 2014 Sep 23.
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Apolipoprotein E;Apo-E;APOE;
Available
Specificity No cross reactivity with other proteins.
Manufacturer - Category
Primary Antibodies
Manufacturer - Isotype
Rabbit IgG
Storage Conditions
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
36 kDa
Calculated Molecular weight
36154 MW
Clonality
Polyclonal
Application Details
Western blot, 0.1-0.5?g/ml

Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml, By Heat

Immunofluorescence, 5 ?g/ml

Flow Cytometry (Fixed), 1-3?g/1x106 cells

ELISA (Cap), 1-5?g/ml
Manufacturer - Gene Name
APOE
Gene Full Name
Apolipoprotein E
Manufacturer - Specificity
No cross reactivity with other proteins.
Background
APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
Immunogen
E.coli-derived human Apolipoprotein E recombinant protein (Position: K19-H317). Human Apolipoprotein E shares 73% and 72% amino acid (aa) sequence identity with mouse and rat Apolipoprotein E, respectively.
Contents
Each vial contains 5 mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category
Cardiovascular, Lipid Metabolism, Lipids/Lipoproteins, Metabolism, Signal Transduction
Protein Name
Apolipoprotein E
Protein Function
Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Subcellular Localization
Secreted.
Description
Boster Bio Anti-Apolipoprotein E/APOE Antibody Picoband® catalog # PB9327. Tested in ELISA, Flow Cytometry, IHC, IF, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity
Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
Sequence Similarities
Belongs to the apolipoprotein A1/A4/E family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug/vial
Available: In stock
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