Manufacturer |
Boster
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Isotype |
IgG |
Format |
Lyophilized |
Applications |
WB, IHC |
Amount |
100 ug/vial |
Host |
Rabbit |
Item no. |
BOS-PB9453 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Manufacturers Research Category |
Adapters, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction, Transmembrane |
Short Description |
Boster Bio Anti-Versican/VCAN Antibody Picoband™ catalog # PB9453. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-Versican/VCAN Antibody Picoband™ catalog # PB9453. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth. |
Gene Name |
VCAN |
Application Details |
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human |
Application Notes |
WB: The detection limit for Versican is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen |
A synthetic peptide corresponding to a sequence at the N-terminus of human Versican, different from the related mouse and rat sequences by two amino acids. |
Purification |
Immunogen affinity purified. |
Cross-reactivity |
No cross-reactivity with other proteins |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene Full Name |
Versican core protein |
Molecular Weight |
372820 MW |
Protein Function |
May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. |
Subcellular Localization |
Secreted, extracellular space, extracellular matrix. |
Tissue Specificity |
Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas. |
Protein Name |
Versican core protein |
Sequence Similarities |
Belongs to the aggrecan/versican proteoglycan family. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.