Manufacturer |
Boster
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Mouse |
Isotype |
IgG |
Format |
Lyophilized |
Applications |
ELISA |
Amount |
100 ug/vial |
Host |
Rabbit |
Item no. |
BOS-A00452-Biotin |
Targets |
LCN2 |
Conjugate/Tag |
Biotin |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Manufacturers Research Category |
Immunoglobulins, Immunology |
Short Description |
Boster Bio Anti-Lipocalin 2/LCN2 Biotinylated Antibody catalog # A00452-Biotin. Tested in ELISA applications. This antibody reacts with Mouse. |
Description |
Boster Bio Anti-Lipocalin 2/LCN2 Biotinylated Antibody catalog # A00452-Biotin. Tested in ELISA applications. This antibody reacts with Mouse. |
Background |
Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor. |
Gene Name |
LCN2 |
Application Details |
ELISA Detection (Matched Antibody Pair), 0.1-0.5μg/ml |
Application Notes |
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen |
E. coli-derived mouse Lipocalin 2 recombinant protein (Position: Q21-N200). |
Purification |
Immunogen affinity purified. |
Cross-reactivity |
No cross-reactivity with other proteins |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene Full Name |
Neutrophil gelatinase-associated lipocalin |
Molecular Weight |
22875 MW |
Protein Function |
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2, 5-dihydroxybenzoic acid (2, 5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.. |
Subcellular Localization |
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. |
Tissue Specificity |
Detected in lung, spleen, uterus, vagina and epididymis.. |
Protein Name |
Neutrophil gelatinase-associated lipocalin |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.