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Anti-SNAP25 Antibody Picoband®

Anti-SNAP25 Antibody Picoband®

Item no.	BOS-A01625
Manufacturer	Boster
Amount	100 ug/vial
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category	Neuroscience Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Antibodies Flow Cytometry Immunohistochemistry Western Blot ELISA Immunofluorescence Immunocytochemistry Cardiovascular Research Heart Failure Hypertension Primary Antibodies Immunostaining Immunochemistry
Type	Antibody Primary
Format	Lyophilized
Applications	WB, FC, IF, IHC, ICC, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Citations	1. Gonelle-Gispert, C.; Halban, P. A.; Niemann, H.; Palmer, M.; Catsicas, S.; Sadoul, K. : SNAP-25a and -25b isoforms are both expressed in insulin-secreting cells and can function in insulin secretion. Biochem. J. 339: 159-165, 1999. 2. Nagy, G.; Reim, K.; Matti, U.; Brose, N.; Binz, T.; Rettig, J.; Neher, E.; Sorensen, J. B. : Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25. Neuron 41: 417-429, 2004. 3. Hodel A (1998). SNAP-25. The International Journal of Biochemistry & Cell Biology 30 (10): 1069–1073. 4. Chapman ER (2002). Synaptotagmin: A Ca2+ sensor that triggers exocytosis?. Nature Reviews Molecular Cell Biology 3: 498–508. 5. Pozzi D, Verderio C, Patti L, Grumelli C, Inverardi F, Frassoni C, Bonanno G, Matteoli M (2004). SNAP-25 modulation of calcium dynamics underlies differences in GABAergic and glutamatergic responsiveness to depolarization. Neuron 41 (4): 599–610.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Synaptosomal-associated protein 25,SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein,SNAP25,SNAP
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	25 kDa
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells ELISA, 0.1-0.5μg/ml
Gene Name	SNAP25
Gene Full Name	synaptosome associated protein 25
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Synaptosome-associated protein of 25, 000 daltons, also known as SNAP-25, is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE (the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa (SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E (BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms (a and b) have been described, which are produced by alternative splicing. It is identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.
Immunogen	E. coli-derived human SNAP25 recombinant protein (Position:M1-L203).
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Cancer, Cancer Metabolism, Cell Type Marker, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Neuron Marker, Neuroscience, Neurotransmission, Pathways and Processes, Protein Trafficking, Secretory Vesicles, Signal Transduction, Synapse Marker, Vesicle Transport
Protein Function	t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Subcellular Localization	Cytoplasm, perinuclear region.
Description	Boster Bio Anti-SNAP25 Antibody Picoband® catalog # A01625. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-A01625-datasheet.pdf

BOS-A01625-safety-datasheet.pdf