Anti-Cysteine protease ATG4A ATG4A Antibody

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Polyclonal

ATG4A

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Marino G., J. Biol. Chem. 278:3671-3678(2003).
Kabeya Y., J. Cell Sci. 117:2805-2812(2004).
Ross M.T., Nature 434:325-337(2005

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1 mg/ml

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Boster Bio Anti-Cysteine protease ATG4A ATG4A Antibody (Catalog # A06539-1). Tested in IHC, IF applications. This antibody reacts with Human, Mouse.

Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.