Comparison

Anti-alpha-Tubulin Tuba1b Antibody European Partner

Item no. BOS-A08382
Manufacturer Boster
Amount 200 ug
Category
Type Antibody
Format Liquid
Applications WB, IF, ELISA
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
Dry ice Yes
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias alpha-Tubulin, ?-tubulin, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3
Available
Manufacturer - Category
Primary Antibodies
Storage Conditions
Store Anti-Tubulin Loading Control Antibody at -20°C prior to opening. Aliquot Loading Control Antibody contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Control Antibody is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Observed Molecular Weight
10951 MW
Manufacturer - Format
Liquid (sterile filtered)
Clonality
Polyclonal
Application notes
Anti-Tubulin Antibody has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
Application Details
ELISA: 1:90, 000
IHC: 1:500 - 1:2, 000
IF Microscopy: 1:500 - 1:2, 000
WB: 1:1, 000 - 1:5, 000
Manufacturer - Gene Name
TUBA1B
Background
Tubulin Loading Control Antibody recognizes microtubules which are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control. Anti-Alpha-tubulin antibody is ideal for investigators involved in cell cycle protein research.
Immunogen
Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Purification
Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein. The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross-reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Concentration
1.1mg/ml by UV absorbance at 280 nm
Manufacturer - Research Category
Cytoskeleton, Cytoskeleton/ECM, Microtubules, Signal Transduction, Tubulin
Short Description
Boster Bio Anti-alpha-Tubulin Tuba1b Antibody (Catalog # A08382). Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Description
Boster Bio Anti-alpha-Tubulin Tuba1b Antibody (Catalog # A08382). Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Predicted Reactivity
Bovine, Chicken

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 200 ug
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?