Manufacturer |
Boster
|
Category |
|
Type |
Antibody |
Specific against |
Human |
Format |
Liquid |
Applications |
WB |
Amount |
100ul |
Host |
Rabbit |
Item no. |
BOS-A17223 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
DNA/RNA, Epigenetics and Nuclear Signaling, Mito. Translation, Ribosome, Translation |
Short Description |
Boster Bio Anti-MRPL50 Antibody (Catalog # A17223). Tested in WB applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-MRPL50 Antibody (Catalog # A17223). Tested in WB applications. This antibody reacts with Human. |
Background |
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.
Ota T., Nat. Genet. 36:40-45(2004). Humphray S.J., Nature 429:369-374(2004). The MGC Project Team; Genome Res. 14:2121-2127(2004). |
Gene Name |
MRPL50 |
Clonality |
Polyclonal |
Concentration |
1 mg/ml |
Contents |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Application Details |
Western blotting: 1:500~1:3000 |
Immunogen |
Synthesized peptide derived from internal of human MRPL50. |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Storage |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Gene Full Name |
39S ribosomal protein L50, mitochondrial |
Molecular Weight |
18325 MW |
Subcellular Localization |
Mitochondrion. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.