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Anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal Antibody

Anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal Antibody

Item no.	BOS-M00953
Manufacturer	Boster
Amount	100 ug
Category	Antibodies Immunohistochemistry Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, IF, IHC
Clone	TOP2A/1361
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Mouse
Isotype	IgG2b Kappa
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	DNA topoisomerase 2-alpha;5.99.1.3;DNA topoisomerase II, alpha isozyme;TOP2A;TOP2;
Available
Manufacturer - Category	Primary Antibodies, Monoclonal Antibodies, 2279
Storage Conditions	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Observed Molecular Weight	174385 MW
Clonality	Monoclonal
Application Details	Immunofluorescence (1-2ug/ml) Western Blot (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
Manufacturer - Gene Name	TOP2A
Gene Full Name	DNA topoisomerase 2-alpha
Background	This monoclonal antibody recognizes a 170kDa protein, which is identified as topoisomerase II is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.
Immunogen	Recombinant human Topoisomerase II alpha fragment (aa1352-1493)
Contents	Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purification	200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G.
Concentration	Purified antibody with BSA and azide at 200ug/ml
Manufacturer - Research Category	Cancer, Chromosome Structure, DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling
Protein Function	Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Subcellular Localization	Nuclear
Short Description	Boster Bio Anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal Antibody (Catalog # M00953). Tested in IF, WB, IHC applications. This antibody reacts with Human, Mouse.
Description	Boster Bio Anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal Antibody (Catalog # M00953). Tested in IF, WB, IHC applications. This antibody reacts with Human, Mouse.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-M00953-datasheet.pdf

BOS-M00953-safety-datasheet.pdf