« Back
Home /
Anti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5)

Anti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5)

Item no.	BOS-M01571-1
Manufacturer	Boster
Amount	100 ug/vial
Category	Neuroscience Neural Lineage Markers Antibodies Western Blot Immunofluorescence Primary Antibodies Cytoskeleton Markers
Type	Antibody Monoclonal
Format	Lyophilized
Applications	WB, FC, IF, IHC, ICC
Clone	2I5
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Mouse
Isotype	IgG1
Sensitivity	>5000 cells
Citations	1.Ghezzi, D., Sevrioukova, I., Invernizzi, F., Lamperti, C., Mora, M., D'Adamo, P., Novara, F., Zuffardi, O., Uziel, G., Zeviani, M. Severe X-linked mitochondrial encephalomyopathy associated with a mutation in apoptosis-inducing factor. Am. J. Hum. Genet. 86: 639-649, 2010. 2.Rinaldi, C., Grunseich, C., Sevrioukova, I. F., Schindler, A., Horkayne-Szakaly, I., Lamperti, C., Landoure, G., Kennerson, M. L., Burnett, B. G., Bonnemann, C., Biesecker, L. G., Ghezzi, D., Zeviani, M., Fischbeck, K. H. Cowchock syndrome is associated with a mutation in apoptosis-inducing factor. Am. J. Hum. Genet. 91: 1095-1102, 2012. 3.Yu, S.-W., Andrabi, S. A., Wang, H., Kim, N. S., Poirier, G. G., Dawson, T. M., Dawson, V. L. Apoptosis-inducing factor mediates poly(ADP-ribose) (PAR) polymer-induced cell death. Proc. Nat. Acad. Sci. 103: 18314-18319, 2006.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	AIFM1 antibody\|AIFM1_HUMAN antibody\|Apoptosis inducing factor 1, mitochondrial antibody\|Apoptosis inducing factor antibody\|Apoptosis inducing factor, mitochondrion associated, 1 antibody\|Apoptosis-inducing factor 1 antibody\|CMTX4 antibody\|COXPD6 antibody\|Harlequin antibody\|Hq antibody\|mAIF antibody\|MGC111425 antibody\|MGC5706 antibody\|mitochondrial antibody\|Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody\|neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody\|PDCD 8 antibody\|PDCD8 antibody\|Programmed cell death 8 (apoptosis inducing factor) antibody\|Programmed cell death 8 antibody\|Programmed cell death 8 isoform 1 antibody\|Programmed cell death 8 isoform 2 antibody\|Programmed cell death 8 isoform 3 antibody\|Programmed cell death protein 8 antibody\|Programmed cell death protein 8 mitochondrial antibody\|Programmed cell death protein 8 mitochondrial precursor antibody\|Striatal apoptosis inducing factor antibody
Available
Specificity	No cross reactivity with other proteins.
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Mouse IgG1
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	70 kDa
Clonality	Monoclonal
Application Details	Western blot, 0.1-0.5?g/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml Immunofluorescence, 2?g/ml Immunocytochemistry/Immunofluorescence, 5?g/ml Flow Cytometry (Fixed), 1-3?g/1x10⁶ cells
Manufacturer - Gene Name	AIFM1
Gene Full Name	apoptosis-inducing factor, mitochondrion-associated, 1
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human AIF, identical to the related mouse and rat sequences.
Contents	Each vial contains 4 mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction
Protein Name	Apoptosis-inducing factor 1, mitochondrial
Protein Function	Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Subcellular Localization	Mitochondrion intermembrane space. Mitochondrion inner membrane. Nucleus. Cytoplasm. Perinuclear region.
Description	Boster Bio Anti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5) catalog # M01571-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-M01571-1-datasheet.pdf

BOS-M01571-1-safety-datasheet.pdf