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Anti-RAB27A Antibody Picoband® (monoclonal, 2F5)

Anti-RAB27A Antibody Picoband® (monoclonal, 2F5)

Item no.	BOS-M01608
Manufacturer	Boster
Amount	100 ug/vial
Category	Antibodies Cell Biology Cytoskeleton Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies small GTPases Rab Subfamily
Type	Antibody Monoclonal
Format	Lyophilized
Applications	WB, FC, IF, IHC, ICC
Clone	2F5
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Mouse
Isotype	IgG2b
Sensitivity	>5000 cells
Citations	1. Aksu, G., Kutukculer, N., Genel, F., Vergin, C., Omowaire, B. Griscelli syndrome without hemophagocytosis in an eleven-year-old girl: expanding the phenotypic spectrum ofRab27A mutations in humans. Am. J. Med. Genet. 116A: 329-333, 2003. 2. Kuroda, T. S., Fukuda, M., Ariga, H., Mikoshiba, K. The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem. 277: 9212-9218, 2002.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram; RAB27A; RAB27
Available
Specificity	No cross reactivity with other proteins.
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Mouse IgG2b
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	27 kDa
Clonality	Monoclonal
Application Details	Western blot, 0.1-0.5?g/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml Immunocytochemistry/Immunofluorescence, 2?g/ml Flow Cytometry (Fixed), 1-3?g/1x10⁶ cells
Manufacturer - Gene Name	RAB27A
Gene Full Name	RAB27A, member RAS oncogene family
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Ras-related protein Rab-27A is a protein that in humans is encoded by the RAB27A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Immunogen	E. coli-derived human RAB27A recombinant protein (Position: L98-K216).
Contents	Each vial contains 4 mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05 mg NaN₃.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Manufacturer - Research Category	Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction
Protein Name	activating transcription factor 1
Protein Function	Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Subcellular Localization	Lysosome. Late endosome. Membrane. Lipid-anchor. Melanosome.
Description	Boster Bio Anti-RAB27A Antibody Picoband® (monoclonal, 2F5) catalog # M01608. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-M01608-datasheet.pdf

BOS-M01608-safety-datasheet.pdf