Comparison

Anti-Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Monoclonal Antibody European Partner

Item no. BOS-M14209-1
Manufacturer Boster
Amount 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, IF, IHC
Clone SPM115
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Monkey (Cynomolgus, Simian), Rabbit (Oryctolagus cuniculus), Chicken (Gallus gallus domesticus), Dog (Canine, Canis lupus familiaris), Cow (Bos taurus)
Host Mouse
Isotype IgG1 Kappa
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Keratin, type II cytoskeletal 1b;Cytokeratin-1B;CK-1B;Keratin-77;K77;Type-II keratin Kb39;KRT77;KRT1B;
Available
Specificity Human,Monkey,Cow,Dog,Rabbit,Mouse,Rat,Chicken,Turtle
Manufacturer - Category
Primary Antibodies, Monoclonal Antibodies, 2279
Storage Conditions
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Observed Molecular Weight
61901 MW
Clonality
Monoclonal
Application Details
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Manufacturer - Gene Name
KRT77
Gene Full Name
Keratin, type II cytoskeletal 1b
Background
This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50' , 48, 46, 45, and 40kDa. MAb SPM116 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Immunogen
Human epidermal keratin
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purification
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G.
Concentration
Purified antibody with BSA and azide at 200ug/ml
Manufacturer - Research Category
Class I, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Keratin, Signal Transduction
Subcellular Localization
Cytoplasmic
Short Description
Boster Bio Anti-Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Monoclonal Antibody (Catalog # M14209-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken, Turtle.
Description
Boster Bio Anti-Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Monoclonal Antibody (Catalog # M14209-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken, Turtle.
Tissue Specificity
Expressed exclusively in skin.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
available

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