Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269

Rabbit IgG

134277 MW

Immunohistochemistry (IHC): 1:500-1:1000 dilution

WB: 1:1000-1:5000 dilution.

Epidermal growth factor receptor

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance (By similarity).

Boster Bio Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269 (Catalog # P00023-5). Tested in IHC, WB applications. This antibody reacts with Human.

Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.