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Anti-Phospho-Merlin (Ser518) NF2 Antibody

Anti-Phospho-Merlin (Ser518) NF2 Antibody

Item no.	BOS-P00279-1
Manufacturer	Boster
Amount	100 ul
Category	Oncology Neuroscience Molecular Targets for Neuroscience Antibodies By Cell Type Carcinoma Hematology Lymphoma Myeloma Melanoma Sarcoma Mesothelioma Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody
Format	Liquid
Applications	WB, IF
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Merlin;Moesin-ezrin-radixin-like protein;Neurofibromin-2;Schwannomerlin;Schwannomin;NF2;SCH;
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	69690 MW
Clonality	Polyclonal
Application Details	Predicted MW: 69kd Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Manufacturer - Gene Name	NF2
Gene Full Name	Merlin
Background	Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Guang-Hui Xiao, et al. (2005) Mol. Cell. Biol ; 25: 2384 - 2394. Hi-Su Yang, et al. (2006) Cancer Res ; 66: 2708 - 2715. R Bohni, et al. (1994) J. Biol. Chem ; 269: 14541 - 14545. Adam J. Ratner, et al. (2001) J. Biol. Chem ; 276: 19267 - 19275.
Immunogen	Peptide sequence around phosphorylation site of serine 518 (R-L-S(p)-M-E) derived from Human Merlin.
Contents	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration	1 mg/ml
Manufacturer - Research Category	Epigenetics and Nuclear Signaling, Intracellular Signaling, Membrane Proteins, Neuroscience, Neurotransmission, Oncoproteins/Suppressors, Signal Transduction, Transcription, Tumor Suppressors
Protein Function	Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
Subcellular Localization	Isoform 1: Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with VPRBP in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions.
Short Description	Boster Bio Anti-Phospho-Merlin (Ser518) NF2 Antibody (Catalog # P00279-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Description	Boster Bio Anti-Phospho-Merlin (Ser518) NF2 Antibody (Catalog # P00279-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-P00279-1-datasheet.pdf

BOS-P00279-1-safety-datasheet.pdf