Anti-Phospho-Beclin-1 (Ser295) BECN1 Antibody

Primary Antibodies

51896 MW

Specific for endogenous levels of the ~60 kDa beclin1 protein phosphorylated at Ser295. Immunolabeling of the beclin-1 band is eliminated in the beclin-1 alanine substitution mutant.

BECN1

Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Akt-mediated phosphorylation of beclin-1 at Ser-295 has been shown to decrease autophagy and promote oncogenesis (Wang et al., 2012).

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Cancer, Cancer Susceptibility, Cell Death, Epigenetics and Nuclear Signaling, Interspecies Interaction, Metabolism, Metabolism Processes, Microbiology, Oncoproteins/Suppressors, Pathways and Processes, Signal Transduction, Transcription, Tumor Suppressors

Plays a central role in autophagy (PubMed:23184933). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3- C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.

Boster Bio Anti-Phospho-Beclin-1 (Ser295) BECN1 Antibody (Catalog # P00327-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Ubiquitous.