Anti-Phospho-Ribosomal Protein S6 (Ser244) Antibody

Primary Antibodies

28681 MW

Specific for endogenous levels of the ~28 kDa rpS6 protein phosphorylated at Ser244. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

RPS6

Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5'-m7 GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-?), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)–Akt–mTOR pathway (Chen and Dittmer 2011).

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

DNA/RNA, Epigenetics and Nuclear Signaling, Ribosome, Translation

May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

Boster Bio Anti-Phospho-Ribosomal Protein S6 (Ser244) Antibody (Catalog # P01567). Tested in WB applications. This antibody reacts with Human, Mouse.