Anti-Phospho-Olig2 (Ser10,13,14) Antibody

Primary Antibodies

32407 MW

Specific for endogenous levels of the ~32 kDa Olig2 phosphorylated at Ser10, Ser 13, and Ser14. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

OLIG2

Olig2 is a well conserved bHLH transcription factor that shows both anti-neural functions and pro-neural functions at different stages in the formation of the oligodendrocyte lineage (Sun et al., 2011). Olig2 is expressed in 100% of the human diffuse gliomas irrespective of grade and required for intracranial tumor formation in a genetically relevant model of malignant glioma (Ligon et al., 2004; Ligon et al., 2007). A developmentally regulated triple serine motif at positions 10, 13 and 14 in the amino terminus is well conserved across species ranging from humans to zebrafish and is essential for Olig2 proliferative function in both normal and malignant neural progenitors (Sun et al., 2011). All three serine residues must be mutated to achieve a strong loss-of-function or gain-of-function phenotype, suggesting that the phosphorylation state of Olig2 represents a significant conformational change in the amino terminus (Sun et al., 2011).

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Cell Type Marker, Epigenetics and Nuclear Signaling, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Stem Cells, Transcription, Transcription Factors

Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

Boster Bio Anti-Phospho-Olig2 (Ser10, 13, 14) Antibody (Catalog # P02247). Tested in WB applications. This antibody reacts with Mouse, Rat.

Expressed specifically in the brain.