Anti-Phospho-Synaptotagmin (Thr202) Syt1 Antibody

Primary Antibodies

47399 MW

Specific for endogenous levels of the ~62 kDa synaptotagmin protein phosphorylated at Thr 202. Immunolabeling is completely eliminated by treatment with ?-phosphatase.

SYT1

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Neuroscience, Neurotransmission, Protein Trafficking, Secretory Vesicles, Signal Transduction, Vesicle Transport

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca (2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca (2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes.

Boster Bio Anti-Phospho-Synaptotagmin (Thr202) Syt1 Antibody (Catalog # P02314). Tested in WB, ICC applications. This antibody reacts with Rat.

Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues.