Anti-Phospho-GABAA Receptor gamma2 (Ser327) GABRG2 Antibody

Primary Antibodies

54162 MW

Specific for endogenous levels of the ~45 kDa GABAA receptor ?2 subunit phosphorylated at Ser327. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

GABRG2

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-AB subtype of receptors. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six ?s, four ?s and four ?s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for ?- and ?-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a ?-subunit is required for benzodiazepine modulation. It has recently been suggested that PKC? regulates the sensitivity of GABA-A ?1?2?2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of ?2 (Qi et al., 2007)

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Amino Acids, Ligand-Gated Ion Channels, Neuroscience, Neurotransmission, Neurotransmitter, Receptors / Channels

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand- gated chloride channel.

Boster Bio Anti-Phospho-GABAA Receptor gamma2 (Ser327) GABRG2 Antibody (Catalog # P02361). Tested in WB applications. This antibody reacts with Mouse, Rat.

Bovine, Canine, Chicken, Human, Primate