Anti-Phospho-Che-1 (AATF) (Ser477) Antibody

Primary Antibodies

63133 MW

Specific for endogenous levels of the ~66 kDa Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

AATF

Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser-477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Apoptosis, Cell Biology, Epigenetics and Nuclear Signaling, Intracellular, Transcription

May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.

Boster Bio Anti-Phospho-Che-1 (AATF) (Ser477) Antibody (Catalog # P03945). Tested in WB applications. This antibody reacts with Human.

Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.