Anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) SLC12A5 Antibody

Primary Antibodies

126184 MW

Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Ser940. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

KCC2

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser-940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane (Lee et al., 2007).

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Neuroscience, Neurotransmission, Potassium Channels, Receptors / Channels

Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl (-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl (-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (By similarity). Involved in the regulation of dendritic spine formation and maturation (PubMed:24668262).

Boster Bio Anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) SLC12A5 Antibody (Catalog # P04629). Tested in WB, IP applications. This antibody reacts with Mouse, Rat.

Brain specific. Detected in neuronal cells.