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Mouse CD30L ELISA Kit PicoKine®

Mouse CD30L ELISA Kit PicoKine®

Item no.	BOS-EK0572
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Hematology Leukemia Lymphoma Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Mesothelioma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Immunostaining Lymphatic System Cancer
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<6 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Tumor necrosis factor ligand superfamily member 8,CD30 ligand,CD30-L,CD153,Tnfsf8,Cd30l,Cd30lg
Available
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	26519 MW
Gene Name	TNFSF8
Gene Full Name	Tumor necrosis factor ligand superfamily member 8
Manufacturer - Specificity	Natural and recombinant mouse CD30L
Background	The human CD30L (CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154 (CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that thisreverse signaling modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.
Immunogen	Expression system for standard: NS0; Immunogen sequence: Q68-D239
Manufacturer - Research Category	Adaptive Immunity, Immunology, T Cells
Protein Name	Tumor necrosis factor ligand superfamily member 8
Protein Function	Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Subcellular Localization	Membrane; Single-pass type II membrane protein.
Description	Mouse CD30L ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfsf8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sample Data	Concentration (pg/ml) \| 0 \| 15.6 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1000 O.D. \| 0.112 \| 0.112 \| 0.133 \| 0.203 \| 0.326 \| 0.594 \| 1.146 \| 2.091
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 35 \| 254 \| 445 \| 38 \| 267 \| 477 Standard deviation \| 2.24 \| 16.51 \| 34.26 \| 2.58 \| 21.09 \| 43.4 CV (%) \| 6.4% \| 6.5% \| 7.7% \| 6.8% \| 7.9% \| 9.1%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 35 \| 30 \| 34 \| 31 \| 32 \| 2.06 \| 6.4% Sample 2 \| 254 \| 260 \| 260 \| 224 \| 249 \| 14.92 \| 5.9% Sample 3 \| 445 \| 507 \| 470 \| 490 \| 478 \| 23.11 \| 4.8% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from rat\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BOS-EK0572.pdf

BOS-EK0572-datasheet.pdf

BOS-EK0572-safety-datasheet.pdf