EFNB2 Ephrin- B2 Mouse Recombinant Protein

EFNB2 Mouse Recombinant produced in Sf9 Baculovirus cells is a single polypeptide chain containing 212 amino acids (29-232) and having a molecular mass of 23.4kDa.
(Molecular size on SDS-PAGE will appear at approximately 28-40KDa).
EFNB2 is fused to 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.

Shipped with wet ice

Store in -20° C for long term storage. After reconstitution, store in 4° C for short term usage within a few days. Avoid freeze-thaw cycles.

Expressed on lateral floor plate cells, specifically on commissural axon segments that have passed through the floor plate. Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk (PubMed:7651410, PubMed:10704386). Expressed in myogenic progenitor cells (PubMed:27446912).