Rabbit Anti-Human ADAM10 (CT) pAb

Proinflammatory cytokine tumor necrosis factor-a, (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1, 2, 4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1, 4). This polyclonal antibody can be used for detection of ADAM10 by Western blot at 1:500 to 1:2000 dilution. Jurkat whole cell lysate can be used as positive control and an approximately 85 kDa band can be detected, which may represent precursor. A 60 kDa faint band was detected in some cell lines including Jurkat, which appears to be the processed mature protein. For research use only.