Human IL-10 ELISA Set

Coating antibodies (2 vials)Product: Monoclonal antibody to human interleukin 10 (IL-10)Production: In vitro using serum free mediumPurification: Ion exchange chromatographyContents: Each vial contains sufficient material for coating of five 96-well ELISA platesBuffer: Prior to lyophilization: 0.25 ml PBS + 125 mM trehaloseApplication: Coating antibody in an ELISA systemReconstitution: Dissolve the contents of one vial by injection of 0.25 ml distilled water into the vial and dilute 100 times in PBS.The total amount of one vial is sufficient for five 96-well ELISA plates (480 determinations, 50 il/well).Detector antibodies (2 vials)Product: Biotinylated monoclonal antibody to human interleukin 10 (IL-10)Isotype: Mouse IgG1Production: In vitro using serum free mediumPurification: Protein A-affinity chromatographyLabeling: With Biotin-7-NHS (N-hydroxysuccinimide)Contents: Each vial contains sufficient material for five 96-well ELISA platesBuffer: Prior to lyophilization: 0.5 ml PBS + 1% BSA + 125 mM trehaloseApplication: Detection antibody in an ELISA systemReconstitution: Dissolve the contents of one vial by injection of 0.5 ml distilled water into the vial and dilute 100 times in PBS +0.5% BSA + 0.05% Tween-20. The total amount of one vial is sufficient for five 96-well ELISA plates (480determinations, 100 il/well).Standards (5 vials)Product: Recombinant human interleukin 10 (IL-10)Application: Cytokine standard in an ELISA systemReconstitution: Dissolve the contents of one vial by injection of 0.5 ml distilled water into the vial and use immediately.GeneralSpecificity: Validated for detecting natural and recombinant human IL-10Sterility: Membrane filtered (0.2 im)Stability: The lyophilized products are stable for more than one year at 4C. After reconstitution, the antibodies are stable for several months at 4C (if kept sterile) or for minimal one year at –20C. The reconstituted standardpreparation should be used immediately.NOT FOR HUMAN USE. FOR RESEARCH ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.