Recombinant Human UE21 His

Lyophilized UBE2I should be stored desiccated below -20C. Upon reconstitution, UBE2I may be stored at 2-4C for 1 week. For future use, store below -20C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Human Ubquitin Conjugating Enzyme 2EI is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 enzyme is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IeBa, and PML without the requirement of an E3 ligase. Recombinant Human Ubiquitin-Conjugating Enzyme E2I is a 19.5 kDa protein containing 171 amino acids. The UBE2I protein contains a 6x His tag and is purified by proprietary chromatographic techniques.

It is recommended to reconstitute the lyophilized UBE2I in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.