Human PAI-1 stable mutant NBD labeled at the reactive center loop *di

Store at -80C. Protect from Light. Stable for 3 years from delivery. For long term use, divide into working aliquots and freeze at -80C. Avoid repeated freeze-thaw cycles.

Plasminogen Activator Inhibitor 1 (PAI-1), also known as Serpin peptidase inhibitor, clade E (SERPINE1), is a member of the serine protease inhibitor (serpin) superfamily. It is the principal inhibitor of Tissue Plasminogen Activator (tPA) and Urokinase (uPA), the activators of Plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium, but is also secreted by other tissue types, such as adipose tissue. Defects in the PAI-1 gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the protein are associated with thrombophilia. The Recombinant Human Serpin E1/PAI-1 Scissor Bond NBD-labeled Stable Point Mutant is produced by the mutagenesis of the P9 serine residue (Ser338) on the reactive center loop of human PAI-1 stable mutant (K154T, Q319L, M354I and N150H) to cysteine. Subsequent incorporation of NBD produced a reporter PAI-1 with extended half life. The properties of this interesting new reagent have not been fully characterized.

>95% active by uPA titration, 1 mole NBD / mole PAI-1

2.5 mg/ml