Recombinant Mouse IL-2

Stable at 2-8C, but best kept desiccated -20C. Upon reconstitution, stable for up to 1 week at 2-8C. For longer term, store in working aliquots below -20C. Avoid repeated freeze/thaw cycles.

Recombinant Mouse Interleukin-2 (IL2) shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch. Mouse and human IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. The 55 kDa IL-2 Ralpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 Rbeta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chaingammac/IL-2 Rgamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 Rbetaandgammac. It drives resting T cells to proliferate and induces IL-2 and IL-2 Ralphasynthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Recombinant Mouse IL2 (IL2) is a single, non-glycosylated polypeptide chain containing 149 amino acids.

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay usingmouse CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of ＞, 5.0 x 106 IU/mg.