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Recombinant Mouse PDGF-BB

Recombinant Mouse PDGF-BB

Manufacturer	Cell Sciences
Category	Oncology Metabolism Diabetes Glucose Homeostasis By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Senescence Astrocytoma By Organ Bladder Cancer Bone Cancer
Type	Proteins
Specific against	Mouse
Amount	2 ug
Host	E.coli
Item no.	CS-CRP138A
Targets	PDGFB, RB1
eClass 6.1	34160400
eClass 9.0	42020190
Available
Biological Activity	Fully biologically active when compared to standard. The activity as measured by the dose-dependent proliferation in mouse 3T3 fibroblasts is 0.2-0.3 ng/ml.
Description	Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-alpha, TGF-beta and EGF. Recombinant mouse PDGF-BB is a non-glycosylated, disulfide-linked homodimer, containing two 109 amino acids chains
Endotoxin Level	Measured by kinetic LAL analysis and is typically <= 1 EU/ug protein.
Formulation	Recombinant mouse PDGF-BB is lyophilized from 10 mM sodium citrate, pH 3.0.
Molecular Weight	24.6 kDa
Reconstitution	Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Storage and Stability	Lyophilized product is very stable at -20C. Reconstituted material should be aliquoted and frozen at -20C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.