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Recombinant Mouse LIF

Recombinant Mouse LIF

Item no.	CS-CSI20150B
Manufacturer	Cell Sciences
Amount	20 ug
Category	Oncology Metabolism Diabetes Glucose Homeostasis Peptides & Proteins Recombinant Proteins Obesity By Organ Heart & Blood Cancer
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
Host	E.coli
Purity	>98% by SDS-PAGE and HPLC analyses.
Sequence	SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Similar products	Lif, Leukemia inhibitory factor
Available
Manufacturer - Category	Biomolecules
Storage Conditions	The lyophilized protein is stable at 2-8C. Upon receipt, store desiccated at -20C. After reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.
Molecular Weight	ca.20 kDa
Description	Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines, and promotion of megakaryocyte production, in vivo . Recombinant Mouse LIF is a single non-glycosylated polypeptide chain containing 180 amino acids.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, + 0.02% Tween 20.
Reconstitution	Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Specificity	>1 x 108 units/mg, where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agarcultures.
Biological Activity	The activity of mouse LIF is determined by the ability to induce differentiation of mouse M1 myeloid leukemic cells. The minimum detectable concentration of mouse LIF in this assay is 0.5 ng/mL.
Endotoxin Level	<0.1 EU/ug as determined by LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 20 ug

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Delivery expected until 9/11/2025

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