Comparison

Recombinant Rat EGF

Item no. CS-CSI20132A
Manufacturer Cell Sciences
Amount 20 ug
Category
Type Proteins
Specific against Rat (Rattus norvegicus)
Host E.coli
Purity 97% by SDS-PAGE and HPLC analyses
Sequence MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products EGF, HOMG4, URG, urogastrone
Available
Manufacturer - Category
Biomolecules
Storage Conditions
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
Molecular Weight
Approximately 6.2 kDa, a single non-glycosylated polypeptide chain containing 54 amino acids, including 3 intramolecular disulfide-bonds.
Description
Epidermal Growth Factor EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor).
Formulation
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed.
Specificity
> 1 x 107 units/mg
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using mouse Balb/c 3T3 cells is less than 0.1 ng/ml
Endotoxin Level
Less than 1EU/ug of rRtEGF as determined by LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20 ug
Available: In stock
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