Synthetic Sincalide

Lyophilized Sincalide although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CCK-8 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Sincalide also called CCK-8 has a molecular formula of C49H62N10O16S3 H-Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH2. Sincalide corresponds to the C-terminal octapeptide of cholecystokinin (CCK) which acts on receptors within the gallbladder wall causing it to contract. Cholecystokinin (CCK) is a hormone originally isolated from pig intestinal mucosa and described as a linear 33-amino acid peptide containing a sulfated tyrosine, which is essential for its biological activity. It has been found in mammals in both the digestive tract and the central nervous system. Among its multiple biological functions, this hormone stimulates pancreatic exocrine secretion, gallbladder contraction, and intestine motility and may also act as a neurotransmitter/neuromodulator in the central nervous system.

It is recommended to reconstitute the lyophilized Sincalide in sterile 18MU-cm water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions