Comparison

Recombinant Protein A

Item no. CS-CSI20280B
Manufacturer Cell Sciences
Amount 20 mg
Category
Type Proteins
Specific against other
Host E.coli
Purity >97% by SDS-PAGE analyses.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Available
Manufacturer - Category
Biomolecules
Storage Conditions
Aliquot and store at -20C. Stable for 2 years at -20C. Avoid repeated freeze-thaw cycles.
Molecular Weight
32 kDa
Description
Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in, E. coli, and functions essentially the same as native Protein A. It consists of 5 IgG binding domains E-D-A-B-C aligned in series. It migrates with an apparent MW = 32 kDa in SDS-PAGE.
Formulation
Lyophilized with no additive.
Reconstitution
Dissolve in distilled water or saline.
Specificity
The interaction between Protein A and IgG is not equivalent for all species. Even within a species, Protein A interacts with some subclasses of IgG and not others. For instance, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1.
Endotoxin Level
Less than 0.1EU/ug of Protein A as determined by LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20 mg
Available: In stock
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