SiHa cells

Disease:
Cervical squamous cell carcinoma
Origin:
This line was established from primary tissue sample obtained from an Asian female patient after surgery. The line is reported to contain an integrated human papillomavirus type 16 genome (HPV-16).
Species:
Homo sapiens
Tissue:
Cervix
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
A hypertriploid human cell line with the modal chromosome number of 71
Patient:
Female, Asian, 55 yrs of age
Medium:
EMEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture:
1:2 to 1:8 (80-90%) using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
II
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Protein Expression: p53 ; pRB +
Isoenzymes: AK-1, 1; ES-D, 2; G6PD, B; GLO-I, 2; Me-2, 1; PGM1, 1; PGM3, 1
DNA Profile (STR): Amelogenin: X; CSF1PO: 12; D13S317: 11; D16S539: 12; D5S818: 9; D7S820: 10; THO1: 6, 9; TPOX: 8; vWA: 14, 17
Related cells by Organ: HEC-59 cells
HeLa cells
SiHa cells
CaSKi cells
C33A cells

Cell Lines