T84 cells

Disease:
Colorectal carcinoma
Origin:
Derived from metastatic lung
Species:
Homo sapiens
Tissue:
Colon
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Tumorigenic; 2n = 46, modal no. 56
Patient:
Male, 72 years of age
Medium:
DMEM/F12 + 10% FBS
Subculture:
1:2 TO 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level: I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Protein Expression: keratin, peptide hormone, neurotransmitter, carcinoembryonic antigen (CEA)
Isoenzymes: G6PD, B; PGM1, 1; PGM3, 1; ES-D, 1; Me-2, 1-2; AK-1, 1; GLO-1, 1-2
DNA Profile (STR): Amelogenin: X; CSF1PO: 10; D13S317: 9; D16S539: 10, 11; D5S818: 12; D7S820: 8, 10; THO1: 6, 9; TPOX: 8, 11; vWA: 17, 18 Mutations in T84 Cells
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: T84 (AddexBio C0009008)
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T84 cells

Cell Lines