HepG2 cells

Disease:
Hepatocellular carcinoma
Origin:
Established from the tumor tissue of a 15-year-old Argentine boy
Species:
Homo sapiens
Tissue:
Liver
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
human hyperdiploid karyotype
Patient:
Male, 15 yrs of age
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Subculture:
1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level: I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Protein Expression: Express insulin, insulin-like growth factor II (IGF II), 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities; decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone
DNA Profile (STR): Amelogenin: X, Y CSF1PO: 10, 11 D13S317: 9, 13 D16S539: 12, 13 D5S818: 11, 13 D7S820: 10 THO1: 9 TPOX: 8, 9 vWA: 17 D3S1358: 15, 16 D21S11: 29, 31 D18S51: 13, 14 Penta E: 15, 20 Penta D: 9, 13 D8S1179: 15, 16/17 FGA: 22, 25 Mutations in HepG2 Cells
Related cells by Organ: Hep3B cells
HepG2 cells

Cell Lines