« Back
Home /
QuantiChrom™ Indole Assay Kit

QuantiChrom™ Indole Assay Kit

Item no.	BAS-DIND-100
Manufacturer	BioAssay Systems
Amount	100 Tests
Quantity options	100 Tests 100 x 100 Tests 20 x 100 Tests
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Shipping Condition	Room temperature
Available
Shipping Temperature	RT
Storage Conditions	4 °C
Description	For quantitative determination of indole and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 100µ L sample. Linear detection range from 3 to 100 µ M indole in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately. Method: OD565nm. Samples: Bacterial growth media and other biological samples. Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 3 µ M.
FAQs	Does the assay work with urine samples The Assay will detect indole in urine samples; however, the reagent also reacts with urobilinogen in the urine. It does not distinguish between the two. Does the assay work with serum samples? The acidity of the reagent often causes serum samples to precipitate which greatly interferes with the assay. Do I need to read the OD immediately after adding the reagent? Yes, we recommend you read the OD shortly after adding the reagent to the samples. The OD of the reaction mixture slowly decreases over time. We would recommend reading the OD within 10 minutes of adding the reagent. For more detailed product information and questions, please feel free to send an inquiry to info@hoelzel.de
Overview	Application: Indole determination in biological samples Key Features: Fast and sensitive. Use of 100uL sample. Linear detection range from 3 to 100 uM indole in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately. Method: OD565nm Samples: Bacterial growth media and other biological samples Species: All Size: 100 tests Detection Limit: 3 uM Shelf Life: 6 months More Details: INDOLE is the primary product of tryptophan breakdown by tryptophanase. The indole test is commonly performed on bacteria to classify them on their ability to break down tryptophan to indole. BioAssay Systems indole assay kit is based on a modified version of Ehlrich's and Kovac's reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample.
Shelf life (Months)	17

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BAS-DIND-100-datasheet.pdf

BAS-DIND-100-safety-datasheet.pdf