H1975 cells

Disease:

Adenocarcinoma; non-small cell lung cancer

Origin:

The line was established in July 1988. The tissue donor was a non-smoker.

Species:

Human

Tissue:

Lung

Morphology:

Epithelial

Properties:

Adherent

Patient:

Female

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium + 10% FBS

Subculture:

Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Split at 1:3 to 1:6. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO