Hs 746T cells

Disease:

Gastric carcinoma

Origin:

This cell line was derived from a metastatic site of left leg.

Species:

Homo sapiens, human

Tissue:

Stomach

Morphology:

Epithelial

Properties:

Adherent, fibroblast

Cytogenic data:

This is a hypertriploid human cell line having the modal chromosome number of 77 occurring in 46% of cells. Cells with higher ploidies were found at 16.6%. The karyotype of this cell line is very complex. Between 45% and 55% of chromosomes in each cell had structural aberrations, including: der(4)t(1; 4) (p13.1; q35); t(15q22q); del(2) (p13); der(7)t(7; ?) (p22; ?) [7p+]; t(7q22q?) and others. The t(15q22q), del(2), and t(7q22q?) (and several others) had two copies in most cells. There were two types of der(7): one was the 7p+ described above; the other, 7p++, was distinctly longer than N1. There were more than ten markers that were present in some cells only. One DM-like chromosome was found in most cells. Normal N7 and N21 were absent. One normal X and one or two normal Y chromosomes were detected.

Patient:

Male, Caucasian, 74 years of age

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. A subcultivation ratio of 1:2 to 1:8 is recommended. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO