Caco-2 Cells

Model of the GI (gastrointestinal) tract, measurement of the Trans-Epithelial/Endothelial Eletrical Resistance (TEER). Caco-2 cells develop high TEER values of up to 2000 cm2 (as measured by CLS using the CellZscope, nanoAnalytics, Münster, Germany).

Caco-2 cells serve as an advanced in vitro model for the human intestinal barrier, primarily due to their differentiation into a cell monolayer that closely resembles the enterocytes lining the small intestine. When culturing the Caco2 cell line on tissue culture filter inserts with polycarbonate filters, Caco-2 cells undergo spontaneous differentiation. The differentiation of Caco2 cells results in the expression of specialized cell types, complete with microvilli, enzymes, and transporters, paralleling the complex features and mechanisms found in an in vivo situation.
In the context of intestinal absorption studies models, Caco-2 cells, which were derived from a human colorectal adenocarcinoma patient, are instrumental due to their ability to develop high TEER values, signifying intact tight junctions and epithelial barrier function. These properties are crucial for assays like the cholesterol efflux assay and investigations into cellular transport, including the movement of lipid nanoparticles and the detection of protein interactions.
Caco-2 cells are pivotal for intestinal absorption studies, providing a reliable in vitro approximation of the intestinal epithelium. Mimicking intestinal enterocytes, these cells facilitate analyses of oral drug absorption by simulating the intestinal barrier. Researchers utilize Caco-2 cells to predict how substances traverse the intestinal mucosa, which is essential for the pharmacokinetic profiling of oral medications. Furthermore, they are a key tool in investigating intestinal cholesterol uptake, homeostasis and transport, which are vital processes for understanding lipid metabolism and associated diseases.
Caco-2 cells remain a cornerstone in colon carcinoma and toxicology research, not only for their relevance to human gastrointestinal studies but also for their role in providing detailed insights into the biliary pathway, the metabolism of xenobiotics within the colon, cancer and toxicology research.

Adherent

72 years

Caucasian

1

Supplement the medium with 10% FBS

After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Yes, in nude mice. Form moderately well differentiated adenocarcinomas consistent with colonic primary (grade II)

1 x 10^4 cells/cm^2 will result in a 90% confluent monolayer in about 4 days

Blood Type O, Rh+, HLA class II negative

Heat stable enterotoxin (Sta, E. coli), epidermal growth factor (EGF), retinoic acid binding protein I and retinol binding protein II, keratin positive.

Me-2, 1, PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 1, G6PD, B.