C127 cells

Disease:
Normal
Origin:
Not specified
Species:
Homo sapiens, human
Tissue:
Gall bladder
Morphology:
Fibroblast
Properties:
Adherent
Cytogenic data:
Not specified
Patient:
Male, 14 weeks gestation
Medium:
AddexBio-formulated DMEM (C0003-01) + 10% FBS
Subculture:
Split sub-confluent cultures (70-80%) at a seed ratio of 1:2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)

Cell Lines