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NS3-CMPK-hLBR1TM-mEGFP growing culture

NS3-CMPK-hLBR1TM-mEGFP growing culture

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Amount	1 cell culture flask
Item no.	CLS-330986
eClass 6.1	32160890
eClass 9.0	32160890
Available
age	30 years
gender	Female
ethnicity	African American
morphology	Fibroblast-like
growthProperties	Adherent
citation	NS3-CMPK-hLBR1TM-mEGFP (CLS catalog number 300986)
biosafetyLevel	1
proteinExpression	H2B-mCherry and DOX inducible NS3-CMPK-hLBR1TM-mEGFP
cultureMedium	DMEM
mediumSupplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose, Add G418 to culture medium at a final concentration of 0.5 mg/ml
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:3 is recommended
fluidRenewal	2 - 3 times per week
freezeMedium	CM-1 (CLS catalog number 800150)
amelogenin	X, X

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

available

Delivery expected until 5/23/2024