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SMAD4 Knockout 293T Cell Line, Heterozygous

SMAD4 Knockout 293T Cell Line, Heterozygous

Manufacturer	Abclonal
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Amount	1 Strips
Item no.	RM01961-1Strips
eClass 6.1	32160890
eClass 9.0	32160890
Available
AntigenSeq	Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO₂ condition. 1. Thaw the vial in 37℃ water bath , and shake it to melt as soon as possible. 2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me- dium and centrifuge 1000rpm for approximately 5 minutes at room temperature. 3. Remove and discard the supernatant. 4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish. 5. Add 8-10mL of complete medium. 6. Incubate the culture at 37℃ incubator with 5% CO₂. 7. A subcultivation ratio of 1:2-1:4 is recommended.
BackGround	This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]
GeneID(Human)	4089
GeneSymbol	SMAD4
Imminogen	Dry ice
ProteinWeight	SMAD4
RecommandDilutionA	1 vial parental cell line and 1 vial knockout cell line
Route	Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
Storage	Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.
protein size	SMAD4

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Amount: 1 Strips

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Delivery expected until 7/2/2024