Comparison

BMPR1B Knockout 293T Cell Line, Homozygous European Partner

Item no. RM02144-1Strips
Manufacturer Abclonal
Amount 1 Strips
Category
Type Cell line
Specific against other
Purity Homozygous knockout
ECLASS 10.1 42040401
ECLASS 11.0 42040401
UNSPSC 41106509
Alias ALK-6, ALK6, AMDD, BDA1D, BDA2, CDw293
Available
Manufacturer - Category
Cell Lines & Derivatives
Storage Conditions
Stored in liquid nitrogen for a long time less than -130°C. Minimizing freeze-thaw cycles.
Background
This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Immunogen
Dry ice
Recommended Dilution
1 vial parental cell line and 1 vial knockout cell line
Protein Size
BMPR1B
Route
Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
AntigenSeq
Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37°C with 5% CO2 condition.
1. Thaw the vial in 37°C water bath , and shake it to melt as soon as possible.
2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me-
        dium and centrifuge 1000rpm for approximately 5 minutes at room temperature.
3. Remove and discard the supernatant.
4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish.
5. Add 8-10mL of complete medium.
6. Incubate the culture at 37°C incubator with 5% CO2.
7. A subcultivation ratio of 1:2-1:4 is recommended.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 Strips
Available: In stock
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