Comparison

CD36 Knockout 293T Cell Line, Homozygous European Partner

Item no. RM02145-1Strips
Manufacturer Abclonal
Amount 1 Strips
Category
Type Cell line
Specific against other
Purity Homozygous knockout
ECLASS 10.1 42040401
ECLASS 11.0 42040401
UNSPSC 41106509
Alias BDPLT10,CHDS7,FAT,GP3B,GP4,GPIV,PASIV,SCARB3
Available
Manufacturer - Category
Cell Lines & Derivatives
Storage Conditions
Stored in liquid nitrogen for a long time less than -130°C. Minimizing freeze-thaw cycles.
Background
The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]
Immunogen
Dry ice
Recommended Dilution
1 vial parental cell line and 1 vial knockout cell line
Protein Size
CD36
Route
Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
Antigen Seq
Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37°C with 5% CO2 condition.
1. Thaw the vial in 37°C water bath , and shake it to melt as soon as possible.
2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me-
        dium and centrifuge 1000rpm for approximately 5 minutes at room temperature.
3. Remove and discard the supernatant.
4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish.
5. Add 8-10mL of complete medium.
6. Incubate the culture at 37°C incubator with 5% CO2.
7. A subcultivation ratio of 1:2-1:4 is recommended.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 Strips
Available: In stock
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