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BCL10 Knockout 293T Cell Line, Homozygous knockout

BCL10 Knockout 293T Cell Line, Homozygous knockout

Item no.	RM02449-1Strips
Manufacturer	Abclonal
Amount	1 Strips
Category	Cell Lines & Cell Culture Cell Lines Cells & Cell lines
Type	Cell line
Specific against	other
Purity	Homozygous knockout
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	CARMEN,CIPER,CLAP,IMD37,c-E10,mE10
Available
Manufacturer - Category	Cell Lines & Derivatives
Storage Conditions	Stored in liquid nitrogen for a long time less than -130°C. Minimizing freeze-thaw cycles.
Background	This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Immunogen	Dry ice
Recommended Dilution	1 vial parental cell line and 1 vial knockout cell line
Protein Size	BCL10
Route	Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
Antigen Seq	Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37°C with 5% CO₂ condition. 1. Thaw the vial in 37°C water bath , and shake it to melt as soon as possible. 2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me- dium and centrifuge 1000rpm for approximately 5 minutes at room temperature. 3. Remove and discard the supernatant. 4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish. 5. Add 8-10mL of complete medium. 6. Incubate the culture at 37°C incubator with 5% CO₂. 7. A subcultivation ratio of 1:2-1:4 is recommended.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 Strips

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Delivery expected until 10/23/2025

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