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IL12A Knockout 293T Cell Line, Homozygous

IL12A Knockout 293T Cell Line, Homozygous

Item no.	RM02534-1strips
Manufacturer	Abclonal
Amount	1 strips
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Purity	Homozygous knockout
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	CLMF, IL-12A, NFSK, NKSF1, P35
Available
Manufacturer - Category	Cell Lines & Derivatives
Storage Conditions	Stored in liquid nitrogen for a long time less than -130°C. Minimizing freeze-thaw cycles.
Background	This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
Immunogen	Dry ice
Recommended Dilution	1 vial parental cell line and 1 vial knockout cell line
Protein Size	IL12A
Route	Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
AntigenSeq	Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37°C with 5% CO₂ condition. 1. Thaw the vial in 37°C water bath , and shake it to melt as soon as possible. 2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me- dium and centrifuge 1000rpm for approximately 5 minutes at room temperature. 3. Remove and discard the supernatant. 4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish. 5. Add 8-10mL of complete medium. 6. Incubate the culture at 37°C incubator with 5% CO₂. 7. A subcultivation ratio of 1:2-1:4 is recommended.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 strips

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